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Amazon rainforest canopy wet season 2024 08:00 local time

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Joseph Byron

Max Planck Institute for Chemistry

Jonathan Williams Group / Atmospheric Chemistry

Outdoor source emission

Pump - sorbent tubes

Sample details

Title Amazon rainforest canopy wet season 2024 08:00 local time

Project ATTO

Date of collection 2024-04-16

Citation Byron, J., Pugliese, G., de A. Monteiro, C. et al. Intense El Niño provokes production of new reactive volatiles as stress defences in Amazon rainforest. Commun Earth Environ 7, 419 (2026). https://doi.org/10.1038/s43247-026-03597-7

Collection method Outdoor source emission

Sampling method

Sampling method Pump - sorbent tubes

Number of sorbents 2

Sorbent type Tenax TA

Sorbent amount 150

Mesh size 35/60

Sorbent type Carbograph 5

Sorbent amount 150

Mesh size 40/60

Sorbent tubes length for TD 89mm

Sorbent tube sampling volume 6132ml

Sorbent tube sampling flow rate 200ml/min

Sorbent tubes storage after sampling Yes

Time between sampling and desorption 33120min

Storage at temperature -4°C

Ozone scrubber Yes

TDU type Markes TD100-xr

Tube desorption stages 1

Desorb time 5min

Desorb temperature 250°C

Trap flow 50ml/min

GC-MS

Focusing trap Markes focusing trap

Focusing trap sorbent-packed Materials emissions

Desorb trap purge time 1min

Trap purge flow 50ml/min

Trap low temperature 30°C

Trap heating rate 100°C/s

Trap high (desorb) temperature 250°C

Trap desorb time 5min

Trap split No

Column type β-DEX™ 120

Column constant flow 1.2ml/min

Length 60m

Inner diameter 0.25mm

Film thickness 0.25μm

Carrier gas He

Temperature ramps

Number of ramps 2

GC initial temperature 50°C

GC initial temperature hold time 5min

GC first temperature ramp rate 1.5°C/min

GC second temperature 110°C

GC second temperature hold time 0min

GC second temperature ramp rate 10°C/min

GC final temperature 220°C

GC final temperature hold time 10 min

Mass Spectrometer detectors Bench-TOF-MS (MARKES)

Scan mass range 41-500

Data rate 4Hz

Ionisation -70eV

Amazon rainforest canopy wet season 2024 05:00 local time

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Joseph Byron

Max Planck Institute for Chemistry

Jonathan Williams Group / Atmospheric Chemistry

Outdoor source emission

Pump - sorbent tubes

Sample details

Title Amazon rainforest canopy wet season 2024 05:00 local time

Project ATTO

Date of collection 2024-04-16

Collection method Outdoor source emission

Sampling method

Sampling method Pump - sorbent tubes

Number of sorbents 2

Sorbent type Tenax TA

Sorbent amount 150

Mesh size 35/60

Sorbent type Carbograph 5

Sorbent amount 150

Mesh size 20/40

Sorbent tubes length for TD 89mm

Sorbent tube sampling volume 6133ml

Sorbent tube sampling flow rate 200ml/min

Sorbent tubes storage after sampling Yes

Time between sampling and desorption 33120min

Storage at temperature -4°C

Ozone scrubber Yes

TDU type Markes TD100-xr

Tube desorption stages 1

Desorb time 5min

Desorb temperature 250°C

Trap flow 50ml/min

GC-MS

Focusing trap Markes focusing trap

Focusing trap sorbent-packed Materials emissions

Desorb trap purge time 1min

Trap purge flow 50ml/min

Trap low temperature 30°C

Trap heating rate 100°C/s

Trap high (desorb) temperature 250°C

Trap desorb time 5min

Trap split Yes

Split flow 2ml/min

Column type β-DEX™ 120

Column constant flow 1.2ml/min

Length 60m

Inner diameter 0.25mm

Film thickness 0.25μm

Carrier gas He

Temperature ramps

Number of ramps 2

GC initial temperature 50°C

GC initial temperature hold time 5min

GC first temperature ramp rate 1.5°C/min

GC second temperature 110°C

GC second temperature hold time 0min

GC second temperature ramp rate 10°C/min

GC final temperature 220°C

GC final temperature hold time 10 min

Mass Spectrometer detectors Bench-TOF-MS (MARKES)

Scan mass range 41-500

Data rate 4Hz

Ionisation -70eV

Amazon rainforest canopy wet season 2024 02:00 local time

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Joseph Byron

Max Planck Institute for Chemistry

Jonathan Williams Group / Atmospheric Chemistry

Outdoor source emission

Pump - sorbent tubes

Sample details

Title Amazon rainforest canopy wet season 2024 02:00 local time

Project ATTO

Date of collection 2026-04-16

Collection method Outdoor source emission

Sampling method

Sampling method Pump - sorbent tubes

Number of sorbents 2

Sorbent type Tenax TA

Sorbent amount 150

Mesh size 35/60

Sorbent type Carbograph 5

Sorbent amount 150

Mesh size 20/40

Sorbent tubes length for TD 89mm

Sorbent tube sampling volume 6133ml

Sorbent tube sampling flow rate 200ml/min

Sorbent tubes storage after sampling Yes

Time between sampling and desorption 33120min

Storage at temperature -4°C

Ozone scrubber Yes

TDU type Markes TD100-xr

Tube desorption stages 1

Desorb time 5min

Desorb temperature 250°C

Trap flow 50ml/min

Split on 0ml/min

GC-MS

Focusing trap Markes focusing trap

Focusing trap sorbent-packed Materials emissions

Desorb trap purge time 1min

Trap purge flow 50ml/min

Trap low temperature 30°C

Trap heating rate 100°C/s

Trap high (desorb) temperature 250°C

Trap desorb time 5min

Trap split No

Column type β-DEX™ 120

Column constant flow 1.2ml/min

Length 60m

Inner diameter 0.25mm

Film thickness 0.25μm

Carrier gas He

Temperature ramps

Number of ramps 2

GC initial temperature 50°C

GC initial temperature hold time 5min

GC first temperature ramp rate 1.5°C/min

GC second temperature 110°C

GC second temperature hold time 0min

GC second temperature ramp rate 10°C/min

GC final temperature 220°C

GC final temperature hold time 10 min

Mass Spectrometer detectors Bench-TOF-MS (MARKES)

Scan mass range 41-500

Data rate 4Hz

Ionisation -70eV

dark chocolate 90%

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Tali Weiss

Weizmann Institute of Science

The department of Brain Sciences

Sample in glass vial

20ml

Dynamic headspace

Sample details

Title dark chocolate 90%

Project Predicting perception of naturalistic odors

Date of collection 2024-10-15

Collection method Sample in glass vial

Collection volume (of the vial) 20ml

Did not weight No

Mass of object sample 3g

Sample storage before sampling? No

Material state Chopped

Suppressing enzyme-driven changes No

Increase headspace sensitivity (“salting out”) No

Sampling method

Sampling method Dynamic headspace

Robotic automation Yes

What platform? Gerstel MPS

Number of sorbents 1

Sorbent type Tenax TA

Mesh size 60/80

Sorbent tubes length for TD 60mm

Sorbent tube sampling volume 20ml

Sorbent tube sampling flow rate 10ml/min

Incubation before sampling Yes

Incubation time 2min

Incubation temperature 35°C

Agitation during the incubation period Yes

Sorbent tubes storage after sampling No

Incubation during sampling Yes

Incubation temperature during sampling 40°C

Dry purge time 1min

Dry purge flow 10ml/min

TDU type Gerstle TDU2

Tube desorption stages 2

Desorb time 2min

Desorb temperature 150°C

Desorb time 2min

Desorb_temperature 250°C

GC-MS

Focusing trap Cooled Injection System

CIS sorbent-packed liner Tenax

CIS low temperature 0°C

CIS high temperature 250°C

Trap split Yes

Split flow 10ml/min

Column type DB-5MS

Column constant flow 1ml/min

Length 30m

Inner diameter 0.25mm

Film thickness 0.5μm

Carrier gas He

Temperature ramps

Number of ramps 1

GC initial temperature 30°C

GC initial temperature hold time 0.5min

GC first temperature ramp rate 10°C/min

GC final temperature 250°C

GC final temperature hold time 1 min

Mass Spectrometer detectors BenchTOF (Sepsolve)

Scan mass range 30-350

Data rate 11Hz

Ionisation -70eV

Ficus tree fruits

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Tali Weiss

Weizmann Institute of Science

The department of Brain Sciences

Sample in glass vial

20ml

Dynamic headspace

Sample details

Title Ficus tree fruits

Project Predicting perception of naturalistic odors

Date of collection 2024-10-13

Collection method Sample in glass vial

Collection volume (of the vial) 20ml

Did not weight Yes

Sample storage before sampling? No

Material state Dried

Increase headspace sensitivity (“salting out”) No

Sampling method

Sampling method Dynamic headspace

Robotic automation Yes

What platform? Gerstel MPS

Number of sorbents 3

Sorbent type Carbopack B

Mesh size 60/80

Sorbent type Carbopack X

Mesh size 40/60

Sorbent type Carboxen 1000

Mesh size 60/80

Sorbent tubes length for TD 60mm

Sorbent tube sampling volume 30ml

Sorbent tube sampling flow rate 10ml/min

Incubation before sampling Yes

Incubation time 3min

Incubation temperature 45°C

Agitation during the incubation period Yes

Sorbent tubes storage after sampling No

Incubation during sampling Yes

Incubation temperature during sampling 40°C

Dry purge time 1min

Dry purge flow 20ml/min

TDU type Gerstle TDU2

Tube desorption stages 2

Desorb time 2min

Desorb temperature 150°C

Desorb time 1min

Desorb_temperature 240°C

GC-MS

Focusing trap Cooled Injection System

CIS sorbent-packed liner Tenax

CIS low temperature 0°C

CIS high temperature 250°C

Trap split Yes

Split flow 50ml/min

Column type DB-5MS

Column constant flow 1ml/min

Length 30m

Inner diameter 0.25mm

Film thickness 0.5μm

Carrier gas He

Temperature ramps

Number of ramps 3

GC initial temperature 30°C

GC initial temperature hold time 0.5min

GC first temperature ramp rate 12°C/min

GC second temperature 90°C

GC second temperature hold time 1min

GC second temperature ramp rate 5°C/min

GC third temperature 150°C

GC third temperature hold time 0.5min

GC third temperature ramp rate 12°C/min

GC final temperature 230°C

GC final temperature hold time 1 min

Mass Spectrometer detectors BenchTOF (Sepsolve)

Scan mass range 30-300

Data rate 11Hz

Ionisation -70eV

Grapes - Scarlota

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Elena Levin

Volcani Institute, Agricultural Research Center

Dr. Itay Maoz group; Postharvest science department

Sample in glass vial

20ml

Solid-Phase Microextraction (SPME)

Sample details

Title Grapes - Scarlota

Project Insights into the chemosensory basis of flavor in table grapes

Date of collection 2019-05-05

Citation https://doi.org/10.1002/jsfa.10102

Collection method Sample in glass vial

Collection volume (of the vial) 20ml

Did not weight No

Mass of object sample 2g

Sample storage before sampling? No

Material state Crushed

Suppressing enzyme-driven changes Yes

Solution saturated NaCl

Incubation duration 1sec

Incubation temperature 25°C

Increase headspace sensitivity (“salting out”) Yes

Salts Sodium chloride

Incubation duration 1sec

Incubation temperature 25°C

Sampling method

Sampling method Solid-Phase Microextraction (SPME)

Robotic automation Yes

What platform? Centri PAL3

Sorbent type DVB/Carbon-WR/PDMS

Sorbent phase thickness 80um

Headspace equilibrium temperature 40°C

Headspace equilibrium time 30min

Extraction mode Yes

Extraction time 30min

Extraction temperature 40°C

Desorption time 5min

Desorption temperature 250°C

Vortex No

GC-MS

Column type HP-5ms

Column constant flow 23.823ml/min

Column constant pressure 5.3171psi

Length 30m

Inner diameter 250mm

Film thickness 0.25μm

Carrier gas He

Temperature ramps

Number of ramps 2

GC initial temperature 40°C

GC initial temperature hold time 1min

GC first temperature ramp rate 5°C/min

GC second temperature 160°C

GC second temperature hold time 2min

GC second temperature ramp rate 10°C/min

GC final temperature 250°C

GC final temperature hold time 1 min

Mass Spectrometer detectors Quadrupole-MS (Agilent)

Scan mass range 40-250

Data rate 50Hz

Ionisation -70eV

Strawberry - Shaked cultivar

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Elena Levin

Volcani Institute, Agricultural Research Center

Dr. Itay Maoz group; Postharvest science department

Sample in glass vial

20ml

Solid-Phase Microextraction (SPME)

Sample details

Title Strawberry - Shaked cultivar

Project Volcani strawberry breeding program

Date of collection 2024-01-16

Collection method Sample in glass vial

Collection volume (of the vial) 20ml

Did not weight No

Mass of object sample 2g

Sample storage before sampling? No

Material state Crushed

Suppressing enzyme-driven changes Yes

Solution saturated NaCl

Incubation duration 1sec

Incubation temperature 25°C

Increase headspace sensitivity (“salting out”) Yes

Salts Sodium chloride

Incubation duration 1sec

Incubation temperature 25°C

Sampling method

Sampling method Solid-Phase Microextraction (SPME)

Robotic automation Yes

What platform? Centri PAL3

Sorbent type DVB/Carbon-WR/PDMS

Sorbent phase thickness 80um

Headspace equilibrium temperature 40°C

Headspace equilibrium time 30min

Extraction mode Yes

Extraction time 30min

Extraction temperature 40°C

Desorption time 5min

Desorption temperature 250°C

Vortex No

GC-MS

Column type HP-5ms

Column constant flow 23.823ml/min

Column constant pressure 5.3171psi

Length 30m

Inner diameter 250mm

Film thickness 0.25μm

Carrier gas He

Temperature ramps

Number of ramps 2

GC initial temperature 40°C

GC initial temperature hold time 1min

GC first temperature ramp rate 5°C/min

GC second temperature 160°C

GC second temperature hold time 2min

GC second temperature ramp rate 10°C/min

GC final temperature 250°C

GC final temperature hold time 1 min

Mass Spectrometer detectors Quadrupole-MS (Agilent)

Scan mass range 40-250

Data rate 50Hz

Ionisation -70eV

Strawberry - Peles cultivar

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Elena Levin

Volcani Institute, Agricultural Research Center

Dr. Itay Maoz group; Postharvest science department

Sample in glass vial

20ml

Solid-Phase Microextraction (SPME)

Sample details

Title Strawberry - Peles cultivar

Project Volcani strawberry breeding program

Date of collection 2024-01-14

Collection method Sample in glass vial

Collection volume (of the vial) 20ml

Did not weight No

Mass of object sample 2g

Sample storage before sampling? No

Material state Crushed

Suppressing enzyme-driven changes Yes

Solution saturated NaCl

Incubation duration 1sec

Incubation temperature 25°C

Increase headspace sensitivity (“salting out”) Yes

Salts Sodium chloride

Incubation duration 1sec

Incubation temperature 25°C

Sampling method

Sampling method Solid-Phase Microextraction (SPME)

Robotic automation Yes

What platform? Centri PAL3

Sorbent type DVB/Carbon-WR/PDMS

Sorbent phase thickness 80um

Headspace equilibrium temperature 40°C

Headspace equilibrium time 30min

Extraction mode Yes

Extraction time 30min

Extraction temperature 40°C

Desorption time 5min

Desorption temperature 250°C

Vortex No

GC-MS

Column type HP-5ms

Column constant flow 23.823ml/min

Column constant pressure 5.3171psi

Length 30m

Inner diameter 250mm

Film thickness 0.25μm

Carrier gas He

Temperature ramps

Number of ramps 2

GC initial temperature 40°C

GC initial temperature hold time 1min

GC first temperature ramp rate 5°C/min

GC second temperature 160°C

GC second temperature hold time 2min

GC second temperature ramp rate 10°C/min

GC final temperature 250°C

GC final temperature hold time 1 min

Mass Spectrometer detectors Quadrupole-MS (Agilent)

Scan mass range 40-250

Data rate 50Hz

Ionisation -70eV

Strawberry - OsoGrande cultivar

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Elena Levin

Volcani Institute, Agricultural Research Center

Dr. Itay Maoz group; Postharvest science department

Sample in glass vial

20ml

Solid-Phase Microextraction (SPME)

Sample details

Title Strawberry - OsoGrande cultivar

Project Volcani strawberry breeding program

Date of collection 2023-12-20

Collection method Sample in glass vial

Collection volume (of the vial) 20ml

Did not weight No

Mass of object sample 2g

Sample storage before sampling? No

Material state Crushed

Suppressing enzyme-driven changes Yes

Solution saturated NaCl

Incubation duration 1sec

Incubation temperature 25°C

Increase headspace sensitivity (“salting out”) Yes

Salts Sodium chloride

Incubation duration 1sec

Incubation temperature 25°C

Sampling method

Sampling method Solid-Phase Microextraction (SPME)

Robotic automation Yes

What platform? Centri PAL3

Sorbent type DVB/Carbon-WR/PDMS

Sorbent phase thickness 80um

Headspace equilibrium temperature 40°C

Headspace equilibrium time 30min

Extraction mode Yes

Extraction time 30min

Extraction temperature 40°C

Desorption time 5min

Desorption temperature 250°C

Vortex No

GC-MS

Column type HP-5ms

Column constant flow 23.823ml/min

Column constant pressure 5.3171psi

Length 30m

Inner diameter 250mm

Film thickness 0.25μm

Carrier gas He

Temperature ramps

Number of ramps 2

GC initial temperature 40°C

GC initial temperature hold time 1min

GC first temperature ramp rate 5°C/min

GC second temperature 160°C

GC second temperature hold time 2min

GC second temperature ramp rate 10°C/min

GC final temperature 250°C

GC final temperature hold time 1 min

Mass Spectrometer detectors Quadrupole-MS (Agilent)

Scan mass range 40-250

Data rate 50Hz

Ionisation -70eV

Strawberry - Orly cultivar

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Elena Levin

Volcani Institute, Agricultural Research Center

Dr. Itay Maoz group; Postharvest science department

Sample in glass vial

20ml

Solid-Phase Microextraction (SPME)

Sample details

Title Strawberry - Orly cultivar

Project Volcani strawberry breeding program

Date of collection 2024-01-25

Collection method Sample in glass vial

Collection volume (of the vial) 20ml

Did not weight No

Mass of object sample 2g

Sample storage before sampling? No

Material state Crushed

Suppressing enzyme-driven changes Yes

Solution saturated NaCl

Incubation duration 1sec

Incubation temperature 25°C

Increase headspace sensitivity (“salting out”) Yes

Salts Sodium chloride

Incubation duration 1sec

Incubation temperature 25°C

Sampling method

Sampling method Solid-Phase Microextraction (SPME)

Robotic automation Yes

What platform? Centri PAL3

Sorbent type DVB/Carbon-WR/PDMS

Sorbent phase thickness 80um

Headspace equilibrium temperature 40°C

Headspace equilibrium time 30min

Extraction mode Yes

Extraction time 30min

Extraction temperature 40°C

Desorption time 5min

Desorption temperature 250°C

Vortex No

GC-MS

Column type HP-5ms

Column constant flow 23.823ml/min

Column constant pressure 5.3171psi

Length 30m

Inner diameter 250mm

Film thickness 0.25μm

Carrier gas He

Temperature ramps

Number of ramps 2

GC initial temperature 40°C

GC initial temperature hold time 1min

GC first temperature ramp rate 5°C/min

GC second temperature 160°C

GC second temperature hold time 2min

GC second temperature ramp rate 10°C/min

GC final temperature 250°C

GC final temperature hold time 1 min

Mass Spectrometer detectors Quadrupole-MS (Agilent)

Scan mass range 40-250

Data rate 50Hz

Ionisation -70eV

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